| Sperm of 180 three year old males of curimbatá, Prochilodus scrofa (Steindachner, 1881), was collected to verify the possibility of utilization of these males more than one time, and to know how long after the hormonal induction would occur the highest production of milt. This experiment was carried out in 1991 and 1992. After sperm was collected, each male was stimulated by the first injection of five IU/g of hCG. Then, milt was collected at 8, 16, 24, 48, 72 and 96 hours before and after the first dose and at 16 hours before and after the second one. Mean volume values were, before hormonal injection, 0.22ml and after two doses of hCG, 106ml. Maximal production occurred between 16 and 24 hours after induction. Mean values of sperm concentration and total... |
